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Using Flow Cytometry and Single-Cell Multiomics for Analyzing COVID-19 Immune Responses


The human immune system exerts coordinated responses capable of overcoming a broad spectrum of pathogenic challenges, including viral infections. Understanding the nature and sequence of these responses paves the way for targeting specific cells for further translational research. 

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High-parameter flow cytometry for deep immune profiling of COVID-19 patients

 

The SARS-CoV-2 virus infection presents a range of clinical manifestations, ranging from mild or no symptoms to moderate to severe respiratory illnesses, such as ARDS (acute respiratory-distress syndrome) and multiorgan failure, to death. The innate immune defense mounted by the body involves pattern recognition receptors (PAR), which activate an array of transcription factors through a signal transduction pathway, ultimately resulting in the secretion of different interferons and chemokines.

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Immunotyping of natural killer (NK) cells in COVID-19 patients using high-parameter flow cytometry

SARS-CoV-2 infection elicits a spectrum of immune responses, activating both the innate and adaptive immune systems. Whether these immune responses are targeted and controlled or misdirected and uncontrolled could determine the outcome of the disease. Recent studies using deep immunoprofiling have indicated that the nature of immune responses can also determine the severity of the disease.

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